Sbieh1, W. Choumane2, M. Matar3 and Q. Al-Rhayeh1
(1) Agricultural Scientific Research Center, Latakia, Syria; (2) Biotechnology Center, University of Tishreen, Latakia, Syria; (3) Faculty of Agricultural Engineering, University of Tishreen, Latakia, Syria.
*Email address of the corresponding author: [email protected]
Received: 3/2/2023; Accepted: 29/11/2023
This study aimed to identify the physiological races of the fungus Fusarium oxysporum f. sp. lycopersici (FOL), the causal agent of tomato Fusarium wilt, in greenhouses along the Syrian coast. The greenhouses in Latakia and Tartous governorates were surveyed during the period 2019-2020. The wilted tomato plant samples were collected during the late stage of plant growth. The pathogenic fungus was isolated from infected plant stems, and the causal agent species was identified according to colony and microscopic characteristics. The pathogenicity of the isolates was confirmed on a susceptible tomato hybrid. The physiological races were identified by the polymerase chain reaction (PCR) analysis of fungus DNA using specific primer sets (SP13 and SP23). The results obtained showed that all isolates belonged to race 1, and based on our knowledge, this is the first report of FOL race 1 along the Syrian coast.
Physiological races, Fusarium wilt, tomato, greenhouses, Syrian coast.
https://doi.org/10.22268/AJPP-001283