Molecular Identification of Fusarium Species Associated with the Tissue Culture Date Palm Offshoots Wilt Disease and Evaluation of Using Silver Nanoparticles and Trichoderma longibrachitum for its Control
M.A. Fayyadh*, A.O. Manea and Y.A. Salih
Plant Protection Department, College of Agriculture, University of Basrah, Iraq. *Email of corresponding author: muamer2010@yahoo.com
Received:12/1/2023; Accepted: 14/6/2023
https://doi.org/10.22268/AJPP-001225
Abstract
This study was conducted during the period 2019-2021 with the aim of isolating and identifying the fungi associated with the tissue culture date palm offshoots wilt disease planted in permanent orchards. Molecular identification of Fusarium isolates based on amplification and nucleotide sequencing of the ITS1-ITS4 gene region showed that isolate F1 and F7 were identical to isolate F. proliferatum with a similarity reached 100 and 98%, respectively; isolates F3 and F4 were identical to isolate F. fujikuroi with a similarity level of 99.8 and 100%, respectively. As for isolate F6, it was 94.8% similar with isolate F. solani. The sequences of isolates were deposited in the US National Center for Biotechnology Information (NCBI) under the numbers OM535259, OM535261, OM535264, OM535265 and OM535266, respectively. The results also showed that silver nanoparticles inhibited the growth of all tested fungi, and the inhibition rate range was 50-70%. On the other hand, the bio-control fungus T. longibrachiatum caused growth inhibition of all tested fungi with an inhibition rate of more than 80%.
Keywords
Date palm, wilt, molecular identification, Nanosiliver particles.